RNA

Part:BBa_K1363601

Designed by: Hanjia Tang   Group: iGEM14_USTC-China   (2014-09-29)

the gate of yes of RNA logic gates

The ribozyme which loses its activity for secondary structure is set in front of a GFP, promoted by a continuous promoter, while a short RNA, the key of yes, is promoted by a Plac. When lac is added, the short RNA is expressed and activate the ribozyme. The cleavage is induced and the expression of protein declines.

Fig.5 The principle of YES gate.
Fig.6 The schematic of YES gate.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 116
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None